pET28a(+)/FbGH17A
(Plasmid
#100911)
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PurposeExpression of the Beta-1,3-Endo-Laminarinase FbGH17A in E.coli
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 100911 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a(+)
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Backbone manufacturerEMD Millipore
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 6602
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFbGH17A
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SpeciesFormosa B
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Insert Size (bp)1233
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GenBank IDAOR29489.1
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Tag
/ Fusion Protein
- His-Tag (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CAGCGGCCTGGTGCCGCGCGGCAGCCATAAAAATAAGCAATCAAATACG
- 3′ sequencing primer CTCAGTGGTGGTGGTGGTGGTGCTCGAGTTAATGGTTCTTTGTGATTTC (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a(+)/FbGH17A was a gift from Jan-Hendrik Hehemann (Addgene plasmid # 100911 ; http://n2t.net/addgene:100911 ; RRID:Addgene_100911)
Map uploaded by the depositor.
