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Addgene

pCS2MT-mbeta-catenin delta5
(Plasmid #100882)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100882 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2MT
  • Backbone manufacturer
    Dave Turner Lab
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ctnnb1
  • Species
    M. musculus (mouse)
  • Mutation
    truncated Ctnnb1 400-666aa
  • Entrez Gene
    Ctnnb1 (a.k.a. Bfc, Catnb, Mesc)
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xMyc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2MT-mbeta-catenin delta5 was a gift from Noah Shroyer (Addgene plasmid # 100882 ; http://n2t.net/addgene:100882 ; RRID:Addgene_100882)

  • For your References section:

    SPDEF Induces Quiescence of Colorectal Cancer Cells by Changing the Transcriptional Targets of beta-catenin. Lo YH, Noah TK, Chen MS, Zou W, Borras E, Vilar E, Shroyer NF. Gastroenterology. 2017 Jul;153(1):205-218.e8. doi: 10.1053/j.gastro.2017.03.048. Epub 2017 Apr 5. 10.1053/j.gastro.2017.03.048 PubMed 28390865
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