pCS2MT-mbeta-catenin delta1
(Plasmid
#100878)
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PurposeMammalian expression of mouse beta-catenin truncation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100878 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2MT
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Backbone manufacturerDave Turner Lab
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCtnnb1
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SpeciesM. musculus (mouse)
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Mutationtruncated Ctnnb1 1-150aa
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Entrez GeneCtnnb1 (a.k.a. Bfc, Catnb, Mesc)
- Promoter CMV
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Tag
/ Fusion Protein
- 6xMyc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2MT-mbeta-catenin delta1 was a gift from Noah Shroyer (Addgene plasmid # 100878 ; http://n2t.net/addgene:100878 ; RRID:Addgene_100878) -
For your References section:
SPDEF Induces Quiescence of Colorectal Cancer Cells by Changing the Transcriptional Targets of beta-catenin. Lo YH, Noah TK, Chen MS, Zou W, Borras E, Vilar E, Shroyer NF. Gastroenterology. 2017 Jul;153(1):205-218.e8. doi: 10.1053/j.gastro.2017.03.048. Epub 2017 Apr 5. 10.1053/j.gastro.2017.03.048 PubMed 28390865