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Addgene

pINDUCER20-Flag-NLS-hSPDEF delta1
(Plasmid #100874)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100874 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pINDUCER20
  • Backbone manufacturer
    Elledge Lab (Addgene #44012)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SPDEF
  • Species
    H. sapiens (human)
  • Mutation
    truncated protein 1-248aa
  • Entrez Gene
    SPDEF (a.k.a. PDEF, bA375E1.3)
  • Promoter TRE2
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pINDUCER20-Flag-NLS-hSPDEF delta1 was a gift from Noah Shroyer (Addgene plasmid # 100874 ; http://n2t.net/addgene:100874 ; RRID:Addgene_100874)

  • For your References section:

    SPDEF Induces Quiescence of Colorectal Cancer Cells by Changing the Transcriptional Targets of beta-catenin. Lo YH, Noah TK, Chen MS, Zou W, Borras E, Vilar E, Shroyer NF. Gastroenterology. 2017 Jul;153(1):205-218.e8. doi: 10.1053/j.gastro.2017.03.048. Epub 2017 Apr 5. 10.1053/j.gastro.2017.03.048 PubMed 28390865
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