pINDUCER20-Flag-hSPDEF WT
(Plasmid
#100873)
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PurposeLentiviral expression of human SPDEF
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 100873 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepINDUCER20
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Backbone manufacturerElledge Lab (Addgene #44012)
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSPDEF
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SpeciesH. sapiens (human)
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Entrez GeneSPDEF (a.k.a. PDEF, bA375E1.3)
- Promoter TRE2
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pINDUCER20-Flag-hSPDEF WT was a gift from Noah Shroyer (Addgene plasmid # 100873 ; http://n2t.net/addgene:100873 ; RRID:Addgene_100873) -
For your References section:
SPDEF Induces Quiescence of Colorectal Cancer Cells by Changing the Transcriptional Targets of beta-catenin. Lo YH, Noah TK, Chen MS, Zou W, Borras E, Vilar E, Shroyer NF. Gastroenterology. 2017 Jul;153(1):205-218.e8. doi: 10.1053/j.gastro.2017.03.048. Epub 2017 Apr 5. 10.1053/j.gastro.2017.03.048 PubMed 28390865