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Addgene

pCMV7.1-Flag-NLS-hSPDEF delta1
(Plasmid #100870)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100870 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3XFlag-CMV7.1
  • Backbone manufacturer
    Sigma-Aldrich
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SPDEF
  • Species
    H. sapiens (human)
  • Mutation
    truncated protein 1-248aa
  • Entrez Gene
    SPDEF (a.k.a. PDEF, bA375E1.3)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV7.1-Flag-NLS-hSPDEF delta1 was a gift from Noah Shroyer (Addgene plasmid # 100870 ; http://n2t.net/addgene:100870 ; RRID:Addgene_100870)

  • For your References section:

    SPDEF Induces Quiescence of Colorectal Cancer Cells by Changing the Transcriptional Targets of beta-catenin. Lo YH, Noah TK, Chen MS, Zou W, Borras E, Vilar E, Shroyer NF. Gastroenterology. 2017 Jul;153(1):205-218.e8. doi: 10.1053/j.gastro.2017.03.048. Epub 2017 Apr 5. 10.1053/j.gastro.2017.03.048 PubMed 28390865
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