pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40
(Plasmid #100855)

• Purpose: AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoter
• Depositing Labs: Douglas Kim, GENIE Project
• Publication: Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi: 10.7554/eLife.12727.

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Backbone

• Vector backbone: pAAV
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: jRGECO1b
• Species: Synthetic
• Promoter: CAG

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40 (p3844)_Q.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was previously available as pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40 (p3844) from the Penn Vector Core. This plasmid was created as part of the GENIE project at Janelia Research Campus.