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Addgene

pAAV.Syn.NES.jRCaMP1a.WPRE.SV40
(Plasmid #100848)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100848 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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AAV1 100848-AAV1 Virus (100 µL at titer ≥ 1×10¹³ vg/mL) and Plasmid. $405
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AAV9 100848-AAV9 Virus (100 µL at titer ≥ 1×10¹³ vg/mL) and Plasmid. $405
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    jRCaMP1a
  • Alt name
    RCaMP1h variant 1488
  • Species
    Synthetic
  • Promoter Synapsin

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was previously available as pAAV.Syn.NES.jRCaMP1a.WPRE.SV40(p3855) from the Penn Vector Core. This plasmid was created as part of the GENIE project at Janelia Research Campus.

Information for AAV1 (Catalog # 100848-AAV1) ( Back to top)

Purpose

Ready-to-use AAV1 particles produced from pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (#100848). In addition to the viral particles, you will also receive purified pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 plasmid DNA.

Syn-driven jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume 100 µL
  • Titer ≥ 1×10¹³ vg/mL
  • Pricing $375 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • Storage Store at -80℃. Thaw just before use and keep on ice.
  • Shipment Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmids encode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
  • Buffer PBS + 0.001% Poloxamer 188
  • Serotype AAV1
  • Purification Iodixanol gradient ultracentrifugation

Biosafety

Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide

Terms and Licenses

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). The specific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for more information.

Addgene Comments

Data submitted about 100848-AAV1 by requesting scientist(s):

Information for AAV9 (Catalog # 100848-AAV9) ( Back to top)

Purpose

Ready-to-use AAV9 particles produced from pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (#100848). In addition to the viral particles, you will also receive purified pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 plasmid DNA.

Syn-driven jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume 100 µL
  • Titer ≥ 1×10¹³ vg/mL
  • Pricing $375 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • Storage Store at -80℃. Thaw just before use and keep on ice.
  • Shipment Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmids encode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
  • Buffer PBS + 0.001% Poloxamer 188
  • Serotype AAV9
  • Purification Iodixanol gradient ultracentrifugation

Biosafety

Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide

Terms and Licenses

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). The specific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 100848 ; http://n2t.net/addgene:100848 ; RRID:Addgene_100848) For viral preps, please replace (Addgene plasmid # 100848) in the above sentence with: (Addgene viral prep # 100848-AAV1) or (Addgene viral prep # 100848-AAV9)

  • For your References section:

    Sensitive red protein calcium indicators for imaging neural activity. Dana H, Mohar B, Sun Y, Narayan S, Gordus A, Hasseman JP, Tsegaye G, Holt GT, Hu A, Walpita D, Patel R, Macklin JJ, Bargmann CI, Ahrens MB, Schreiter ER, Jayaraman V, Looger LL, Svoboda K, Kim DS. eLife. 2016 Mar 24;5. pii: e12727. doi: 10.7554/eLife.12727. 10.7554/eLife.12727 PubMed 27011354
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