pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40
(Plasmid
#100831)
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PurposeAAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100831 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCaMPARI
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SpeciesSynthetic
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Insert Size (bp)1308
- Promoter CAG
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was previously available as pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40 (p4090) from the Penn Vector Core.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40 was a gift from Loren Looger & Eric Schreiter (Addgene plasmid # 100831 ; http://n2t.net/addgene:100831 ; RRID:Addgene_100831) -
For your References section:
Neural circuits. Labeling of active neural circuits in vivo with designed calcium integrators. Fosque BF, Sun Y, Dana H, Yang CT, Ohyama T, Tadross MR, Patel R, Zlatic M, Kim DS, Ahrens MB, Jayaraman V, Looger LL, Schreiter ER. Science. 2015 Feb 13;347(6223):755-60. doi: 10.1126/science.1260922. 10.1126/science.1260922 PubMed 25678659