Myc-Bnip3FL
(Plasmid #100796)

• Purpose: Mammalian expression of Bnip3 full length splice variant
• Depositing Lab: Joseph Gordon
• Publication: Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;61(5):583-90. doi: 10.1097/MPG.0000000000000876.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mus Bnip3
• Species: M. musculus (mouse)
• Insert Size (bp): 564
• Entrez Gene: Bnip3 (a.k.a. Nip3)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry