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Addgene

HA-mus-Bnip3_deltaExon3
(Plasmid #100793)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100793 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse-Bnip3_deltaExon3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    228
  • Mutation
    Exon3 deletion
  • Entrez Gene
    Bnip3 (a.k.a. Nip3)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T8
  • 3′ sequencing primer Sp7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-mus-Bnip3_deltaExon3 was a gift from Joseph Gordon (Addgene plasmid # 100793 ; http://n2t.net/addgene:100793 ; RRID:Addgene_100793)

  • For your References section:

    Human Milk Fortification Increases Bnip3 Expression Associated With Intestinal Cell Death In Vitro. Diehl-Jones W, Archibald A, Gordon JW, Mughal W, Hossain Z, Friel JK. J Pediatr Gastroenterol Nutr. 2015 Nov;61(5):583-90. doi: 10.1097/MPG.0000000000000876. 10.1097/MPG.0000000000000876 PubMed 26505960
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