HA-human-BNIP3 delta Exon(2+3)
(Plasmid #100783)

• Purpose: Mammalian expression of human-BNIP3 delta Exon2+3 splice variant
• Depositing Lab: Joseph Gordon
• Publication: Gordon Lab plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human-BNIP3 delta Exon(2+3)
• Species: H. sapiens (human)
• Insert Size (bp): 95
• Mutation: Exon2 and Exon3 deletion
• Entrez Gene: BNIP3 (a.k.a. HABON, NIP3)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry