HA-human-BNIP3 delta Exon2
(Plasmid #100782)

• Purpose: Mammalian expression of BNIP3 delta Exon2 splice variant
• Depositing Lab: Joseph Gordon
• Publication: Field et al Cell Death Discov. 2018 Sep 21;4:37. doi: 10.1038/s41420-018-0104-z. eCollection 2018.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human-BNIP3 delta Exon2
• Species: H. sapiens (human)
• Insert Size (bp): 180
• Mutation: Exon2 deletion
• Entrez Gene: BNIP3 (a.k.a. HABON, NIP3)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry