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pLenti-Mito-Car-GECO
(Plasmid #100765)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100765 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-puro
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7067
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lenti-Mito-Car-GECO
  • Species
    Synthetic
  • Insert Size (bp)
    1458
  • Mutation
    R-GECO1: E163V/I166V/V174T/M176I/F222I/A302P
  • Promoter CMV
  • Tag / Fusion Protein
    • a duplex of the mitochondrial targeting signal of cytochrome c oxidase subunit VIII (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-fwd
  • 3′ sequencing primer V5-rev and BGH-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

sub-cloned from Addgene CMV-mito-CAR-GECO1 #46022

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-Mito-Car-GECO was a gift from Joseph Gordon (Addgene plasmid # 100765 ; http://n2t.net/addgene:100765 ; RRID:Addgene_100765)

  • For your References section:

    Myocardin regulates mitochondrial calcium homeostasis and prevents permeability transition. Mughal W, Martens M, Field J, Chapman D, Huang J, Rattan S, Hai Y, Cheung KG, Kereliuk S, West AR, Cole LK, Hatch GM, Diehl-Jones W, Keijzer R, Dolinsky VW, Dixon IM, Parmacek MS, Gordon JW. Cell Death Differ. 2018 Mar 6. pii: 10.1038/s41418-018-0073-z. doi: 10.1038/s41418-018-0073-z. 10.1038/s41418-018-0073-z [pii] PubMed 29511336
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