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PurposeMammalian expression of Fluorescent Nix
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100763 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
- Backbone size w/o insert (bp) 6078
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBCL2 interacting protein 3 like
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SpeciesH. sapiens (human)
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Insert Size (bp)660
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Entrez GeneBNIP3L (a.k.a. BNIP3a, NIP3L, NIX)
- Promoter CMV
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Tag
/ Fusion Protein
- DsRed (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DsRed-Nix was a gift from Joseph Gordon (Addgene plasmid # 100763 ; http://n2t.net/addgene:100763 ; RRID:Addgene_100763)