Myc-Nix-CytoB5
(Plasmid #100756)

• Purpose: Mammalian expression of ER targeted Nix
• Depositing Lab: Joseph Gordon
• Publication: Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1038/s41418-018-0073-z. doi: 10.1038/s41418-018-0073-z.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nix-CytoB5
• Species: H. sapiens (human)
• Insert Size (bp): 663
• Mutation: TM domain replaced by CytoB
• Entrez Gene: BNIP3L (a.k.a. BNIP3a, NIP3L, NIX)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry