CD8-GFP-FKBP
(Plasmid #100751)

• Purpose: Expression of GFP-FKBP at the plasma membrane in mammalian cells. Plasma membrane targeting is via CD8 alpha chain.
• Depositing Lab: Stephen Royle
• Publication: Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 10.1038/s41467-018-04993-0.

Backbone

• Vector backbone: pmCherry-N1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CD8A
• Alt name: CD8 alpha chain
• Species: H. sapiens (human)
• Mutation: Truncated
• Entrez Gene: CD8A (a.k.a. CD8, CD8alpha, Leu2, p32)
• Promoter: CMV
• Tags / Fusion Proteins:
  • GFP (C terminal on insert)
  • FKBP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: NheI (unknown if destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• Supplemental Documents:
  • CD8-GFP-FKBP.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry