FRB-mCherry-FTH1
(Plasmid #100749)

• Purpose: Part of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tag
• Depositing Lab: Stephen Royle
• Publication: Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 10.1038/s41467-018-04993-0.

Backbone

• Vector backbone: pmCherry-C1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ferritin heavy chain 1
• Alt name: Ferritin heavy chain
• Species: H. sapiens (human)
• Entrez Gene: FTH1 (a.k.a. FHC, FTH, FTHL6, HFE5, NBIA9, PIG15, PLIF)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FRB (N terminal on insert)
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRII (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• Supplemental Documents:
  • FRB-mCherry-FTH1.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry