MBP-CHC(1-1074)-His6
(Plasmid #100748)

• Purpose: Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.
• Depositing Lab: Stephen Royle
• Publication: Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.201702188. doi: 10.1083/jcb.201702188.

Backbone

• Vector backbone: pMALPre-His
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CLTC
• Alt name: CHC
• Alt name: CHC17
• Alt name: clathrin heavy chain
• Species: H. sapiens (human)
• Mutation: Residues 1-1074
• Entrez Gene: CLTC (a.k.a. CHC, CHC17, CLH-17, CLTCL2, Hc, MRD56)
• Tags / Fusion Proteins:
  • MBP (N terminal on insert)
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: BamHI/BglII (destroyed during cloning)
• 5′ sequencing primer: MBP-F

Resource Information

• Supplemental Documents:
  • MBP-CHC(1-1074)-His6.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry