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Addgene

GST-beta2(616-951)Double
(Plasmid #100746)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100746 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6P-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AP2B1
  • Alt name
    beta2 adaptin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1008
  • Mutation
    Long isoform with deletion of clathrin binding motif and Y815A
  • GenBank ID
    BC006201 NM_001030006.1
  • Entrez Gene
    AP2B1 (a.k.a. ADTB2, AP105B, AP2-BETA, CLAPB1)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer pGEX 5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The mutation numbering in this plasmid refers to the shorter isoform. The mutation in the longer isoform of AP2B1, which is contained in this plasmid, is Y829A.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-beta2(616-951)Double was a gift from Stephen Royle (Addgene plasmid # 100746 ; http://n2t.net/addgene:100746 ; RRID:Addgene_100746)

  • For your References section:

    New tools for "hot-wiring" clathrin-mediated endocytosis with temporal and spatial precision. Wood LA, Larocque G, Clarke NI, Sarkar S, Royle SJ. J Cell Biol. 2017 Sep 27. pii: jcb.201702188. doi: 10.1083/jcb.201702188. 10.1083/jcb.201702188 PubMed 28954824
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