GST-beta2(616-951)
(Plasmid #100743)

• Purpose: Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form
• Depositing Lab: Stephen Royle
• Publication: Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.201702188. doi: 10.1083/jcb.201702188.

Backbone

• Vector backbone: pGEX-6P-1
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AP2B1
• Alt name: beta2 adaptin
• Species: H. sapiens (human)
• Insert Size (bp): 1008
• Mutation: Long isoform
• GenBank ID: BC006201 NM_001030006.1
• Entrez Gene: AP2B1 (a.k.a. ADTB2, AP105B, AP2-BETA, CLAPB1)
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: pGEX 5'

Resource Information

• Supplemental Documents:
  • GST-beta2(616-951).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry