FKBP-beta2(616-951)-GFP Y215A
(Plasmid #100727)

• Purpose: To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis
• Depositing Lab: Stephen Royle
• Publication: Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.201702188. doi: 10.1083/jcb.201702188.

Backbone

• Vector backbone: pEGFP-N1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AP2B1
• Alt name: beta2 adaptin
• Species: H. sapiens (human)
• Insert Size (bp): 1008
• Mutation: Long isoform Y215A mutation (Y815A in full length)
• GenBank ID: BC006201 NM_001030006.1
• Entrez Gene: AP2B1 (a.k.a. ADTB2, AP105B, AP2-BETA, CLAPB1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FKBP (N terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • FKBP-beta2(616-951)-GFP Y215A.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The mutation numbering in this plasmid refers to the shorter isoform. The mutation in the longer isoform of AP2B1, which is contained in this plasmid, is Y829A.