Skip to main content
Addgene

B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)
(Plasmid #100716)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100716 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    B52 plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette
  • Total vector size (bp) 2634
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sgSTOP targeting ATP1A1 (cloned using BsmBI)
  • gRNA/shRNA sequence
    sgSTOP ATP1A1: GATCCAAGCTGCTACAGAAG
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer GAGGTACCTCGAGGAATTCTCTAGA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette) was a gift from Alberto Ciccia (Addgene plasmid # 100716 ; http://n2t.net/addgene:100716 ; RRID:Addgene_100716)

  • For your References section:

    CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons. Billon P, Bryant EE, Joseph SA, Nambiar TS, Hayward SB, Rothstein R, Ciccia A. Mol Cell. 2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 10.1016/j.molcel.2017.08.008. 10.1016/j.molcel.2017.08.008 PubMed 28890334
Related items:
Commonly requested with: