YCe3777 HC_Kan_Chimeric-5'LTR_p1
(Plasmid
#100704)
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Purposepart designed to occupy position 1 of EMMA. Functional category: Long Terminal Repeat
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100704 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSMART HC_Kan
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLentiviral chimeric 5' LTR
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Chimeric 5'LTR for lentiviral vectors :It contains an RSV promoter, a truncated 5'LTR and other lentiviral elements such as Phi element, cPPT/CTS and RRE. Note: it contains an internal BsaI site that has not been removed to not alter the original sequence
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YCe3777 HC_Kan_Chimeric-5'LTR_p1 was a gift from Yizhi Cai (Addgene plasmid # 100704 ; http://n2t.net/addgene:100704 ; RRID:Addgene_100704) -
For your References section:
EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y. ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. 10.1021/acssynbio.7b00016 PubMed 28418644