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Addgene

YCe3736_HC_Amp_ccdB_receiver vector
(Plasmid #100637)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100637 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSMART HC_Amp
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Receiver vector for EMMA. It is the plasmid used as receiver vector in the BsmBI golden gate reaction of EMMA and therefore provides the backbone of the resulting expression vector. It contains a ccdB gene which is replaced by the assembling parts.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YCe3736_HC_Amp_ccdB_receiver vector was a gift from Yizhi Cai (Addgene plasmid # 100637 ; http://n2t.net/addgene:100637 ; RRID:Addgene_100637)

  • For your References section:

    EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y. ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. 10.1021/acssynbio.7b00016 PubMed 28418644
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