AAEL005635-Cas9
(Plasmid #100608)

• Purpose: The promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti
• Depositing Lab: Omar Akbari
• Publication: Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. pii: 201711538. doi: 10.1073/pnas.1711538114.

Backbone

• Vector backbone: PiggyBac
• Backbone size w/o insert (bp): 5246
• Total vector size (bp): 15788
• Vector type: Insect Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: AAEL005635-hspcas9-GFP
• Species: Aedes aegypti, Streptococcus pyogenes
• Insert Size (bp): 8768
• Promoter: AAEL005635
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGACGGTCACGGCGGGCATGTCGACGCGGCCGCATGTGAATCAACATTAGGTTGTCATGA
• 3′ sequencing primer: AAAAGTTGGTGGTGGGGAGGCCACCGAGTATGGGCGCGCCCCGGCCGTTAACTCGAATCG

Gene/Insert 2

• Gene/Insert name: Opie2-dsRed
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 926
• Promoter: Opie2
• Tag / Fusion Protein:
  • dsRED (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GGATAGCGATTCGAGTTAACGGCCGGGgcgcgcccatactcggtggcctccccacca
• 3′ sequencing primer: AACATTGTCAGATCCGAGATCGGCCGGCCTAGAAGCTTTAAGATACATTGATGAGTTTGGAC

Resource Information

• Supplemental Documents:
  • AAEL005635-Cas9.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry