pcDNA3.1-DTYMK
(Plasmid #100546)

• Purpose: mammalian expression of deoxythymidylate kinase
• Depositing Lab: Yuh-Shan Jou
• Publication: Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751. doi: 10.18632/oncotarget.20774. eCollection 2017 Sep 29.

Backbone

• Vector backbone: pCDNA3.1+
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: deoxythymidylate kinase
• Alt name: CDC8, PP3731
• Alt name: TMPK, TYMK, DTYMK
• Species: H. sapiens (human)
• Insert Size (bp): 639
• Entrez Gene: DTYMK (a.k.a. CDC8, PP3731, TMPK, TYMK)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry