AV09_pDonor.EP.S1.TS
(Plasmid #100297)

• Purpose: AAVS1-targeting expression plasmid
• Depositing Lab: Manuel Goncalves
• Publication: Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 10.1038/s41467-017-00687-1.

Backbone

• Vector backbone: pBR322
• Total vector size (bp): 8041
• Vector type: Mammalian Expression, CRISPR, Synthetic Biology
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PuroR.T2A.EGFP expression unit
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 2185

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Combine this donor plasmid with SpCas9 or SpCas9.D10A with Addgene plasmid 41818 gRNA_AAVS1-T2; Mali et al. (named pgRNA.S1 in Chen et al. 2017) for inducing homology-directed AAVS1 targeting. This donor plasmid has its targeting module flanked by the target site (TS) for gRNA.S1: 5’-CTGTCCCCTCCACCCCACAGTggggccactagggacaggattggTGACAGA-3’.