AV11_pDonor.EP.S1
(Plasmid #100296)

• Purpose: AAVS1-targeting expression plasmid
• Depositing Lab: Manuel Goncalves
• Publication: Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 10.1038/s41467-017-00687-1.

Backbone

• Vector backbone: pBR322
• Total vector size (bp): 7881
• Vector type: Mammalian Expression, CRISPR, Synthetic Biology
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PuroR.T2A.EGFP expression unit
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 2181

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Combine this donor plasmid with SpCas9 or SpCas9.D10A with Addgene plasmid 41818 gRNA_AAVS1-T2; Mali et al. (named pgRNA.S1 in Chen et al.) for homology-directed AAVS1 targeting.