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Addgene

AX44_pS.Donor.S1
(Plasmid #100289)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100289 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Total vector size (bp) 3333
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology ; Human Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    18 bp RFLP (MluI+KspAI)
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    617

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Combine this donor plasmid with SpCas9 or SpCas9.D10A with Addgene plasmid 41818 gRNA_AAVS1-T2; Mali et al. (named pgRNA.S1 in Chen et al.) for homology-directed AAVS1 targeting.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AX44_pS.Donor.S1 was a gift from Manuel Goncalves (Addgene plasmid # 100289 ; http://n2t.net/addgene:100289 ; RRID:Addgene_100289)

  • For your References section:

    In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting. Chen X, Janssen JM, Liu J, Maggio I, 't Jong AEJ, Mikkers HMM, Goncalves MAFV. Nat Commun. 2017 Sep 22;8(1):657. doi: 10.1038/s41467-017-00687-1. 10.1038/s41467-017-00687-1 PubMed 28939824
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