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Addgene

AL62_pDonor.DMD.TS
(Plasmid #100287)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100287 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology ; Human Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EGFP expression unit
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    6301
  • Entrez Gene
    DMD (a.k.a. BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AL62_pDonor.DMD.TS was a gift from Manuel Goncalves (Addgene plasmid # 100287 ; http://n2t.net/addgene:100287 ; RRID:Addgene_100287)

  • For your References section:

    In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting. Chen X, Janssen JM, Liu J, Maggio I, 't Jong AEJ, Mikkers HMM, Goncalves MAFV. Nat Commun. 2017 Sep 22;8(1):657. doi: 10.1038/s41467-017-00687-1. 10.1038/s41467-017-00687-1 PubMed 28939824
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