pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA
(Plasmid
#100279)
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PurposeIn vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100279 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKdm6b
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Alt nameJmjd3
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SpeciesM. musculus (mouse)
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MutationMutated catalytic domain (1025aa-End) (please see depositor comments below)
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Entrez GeneKdm6b (a.k.a. 1700064E03Rik, Jmjd3)
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Tag
/ Fusion Protein
- Flag epitope tag (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that mutation H1388A was found during Addgene's quality control when we compared the sequence for Kdm6b to Genbank sequence NM_001017426.1. The depositor noted this mutation is correct (same function as H1390A) and this plasmid should function as described in the publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA was a gift from Yi Zhang (Addgene plasmid # 100279 ; http://n2t.net/addgene:100279 ; RRID:Addgene_100279) -
For your References section:
Maternal H3K27me3 controls DNA methylation-independent imprinting. Inoue A, Jiang L, Lu F, Suzuki T, Zhang Y. Nature. 2017 Jul 19. doi: 10.1038/nature23262. 10.1038/nature23262 PubMed 28723896