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Addgene

pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA
(Plasmid #100279)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100279 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kdm6b
  • Alt name
    Jmjd3
  • Species
    M. musculus (mouse)
  • Mutation
    Mutated catalytic domain (1025aa-End) (please see depositor comments below)
  • Entrez Gene
    Kdm6b (a.k.a. 1700064E03Rik, Jmjd3)
  • Tag / Fusion Protein
    • Flag epitope tag (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that mutation H1388A was found during Addgene's quality control when we compared the sequence for Kdm6b to Genbank sequence NM_001017426.1. The depositor noted this mutation is correct (same function as H1390A) and this plasmid should function as described in the publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA was a gift from Yi Zhang (Addgene plasmid # 100279 ; http://n2t.net/addgene:100279 ; RRID:Addgene_100279)
  • For your References section:

    Maternal H3K27me3 controls DNA methylation-independent imprinting. Inoue A, Jiang L, Lu F, Suzuki T, Zhang Y. Nature. 2017 Jul 19. doi: 10.1038/nature23262. 10.1038/nature23262 PubMed 28723896