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pGEX4T3-Stx3-L77A/L80A
(Plasmid #100273)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100273 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T3
  • Backbone manufacturer
    GE Lifesciences
  • Backbone size w/o insert (bp) 4968
  • Total vector size (bp) 5838
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Stx3-1-265-L77A/L80A
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    870
  • Mutation
    No transmembrane domain; residues 4-264 present, changed both L77 and L80 to alanines
  • Entrez Gene
    Stx3 (a.k.a. Stx3a)
  • Promoter Tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T3-Stx3-L77A/L80A was a gift from Thomas Weimbs (Addgene plasmid # 100273 ; http://n2t.net/addgene:100273 ; RRID:Addgene_100273)

  • For your References section:

    The H(abc) domain of syntaxin 3 is a ubiquitin binding domain. Giovannone AJ, Reales E, Bhattaram P, Nackeeran S, Monahan AB, Syed R, Weimbs T. Sci Rep. 2020 Dec 7;10(1):21350. doi: 10.1038/s41598-020-78412-0. 10.1038/s41598-020-78412-0 PubMed 33288783
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