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Addgene

pEV_S
(Plasmid #100266)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100266 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDSW204
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 18830
  • Modifications to backbone
    Added cos site for plasmid stability with large inserts.
  • Vector type
    Bacterial Expression, Synthetic Biology ; Expression, pBR322

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    In minimal medium, grow at 30C.
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    entD
  • Species
    Escherichia coli
  • GenBank ID
    ID 945194
  • Entrez Gene
    entD (a.k.a. b0583, ECK0575)

Gene/Insert 2

  • Gene/Insert name
    entC
  • Species
    Escherichia coli
  • GenBank ID
    ID 945511

Gene/Insert 3

  • Gene/Insert name
    entE
  • Species
    Escherichia coli
  • GenBank ID
    ID 947426

Gene/Insert 4

  • Gene/Insert name
    entB
  • Species
    Escherichia coli
  • GenBank ID
    ID 946178

Gene/Insert 5

  • Gene/Insert name
    entA
  • Species
    Escherichia coli
  • GenBank ID
    ID 945284

Gene/Insert 6

  • Gene/Insert name
    vibH
  • Species
    Vibrio cholerae
  • GenBank ID
    ID 2615318

Gene/Insert 7

  • Gene/Insert name
    vibF
  • Species
    Vibrio cholerae
  • GenBank ID
    ID 2614958

Gene/Insert 8

  • Gene/Insert name
    cos

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing results found an A57E mutation in entE and a Q182R mutation in entA. Plasmid functions as described.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEV_S was a gift from Timothy Lu (Addgene plasmid # 100266 ; http://n2t.net/addgene:100266 ; RRID:Addgene_100266)

  • For your References section:

    An Engineered Synthetic Pathway for Discovering Nonnatural Nonribosomal Peptides in Escherichia coli. Cleto S, Lu TK. MBio. 2017 Oct 10;8(5). pii: e01474-17. doi: 10.1128/mBio.01474-17. 10.1128/mBio.01474-17 PubMed 29018120
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