Keasling-2187
(Plasmid
#100167)
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PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 100167 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAM43 derivative
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMevT and MBIS operon
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SpeciesSynthetic
- Promoter lacUV5
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Keasling-2187 was a gift from Jay Keasling (Addgene plasmid # 100167 ; http://n2t.net/addgene:100167 ; RRID:Addgene_100167) -
For your References section:
Optimization of the mevalonate-based isoprenoid biosynthetic pathway in Escherichia coli for production of the anti-malarial drug precursor amorpha-4,11-diene. Anthony JR, Anthony LC, Nowroozi F, Kwon G, Newman JD, Keasling JD. Metab Eng. 2009 Jan;11(1):13-9. doi: 10.1016/j.ymben.2008.07.007. Epub 2008 Aug 12. 10.1016/j.ymben.2008.07.007 PubMed 18775787
