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Addgene

pET26-CNTN149L
(Plasmid #100164)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100164 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET26
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Concentrative nucleoside transporter
  • Alt name
    EGZ45206
  • Species
    Neisseria wadsworthii
  • Insert Size (bp)
    1278
  • Mutation
    N149L
  • Promoter T7
  • Tag / Fusion Protein
    • MBP-His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvrII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CAGATGTCCGCTTTCTGGTATG
  • 3′ sequencing primer T7_reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET26-CNTN149L was a gift from Seok-Yong Lee (Addgene plasmid # 100164 ; http://n2t.net/addgene:100164 ; RRID:Addgene_100164)

  • For your References section:

    Visualizing multistep elevator-like transitions of a nucleoside transporter. Hirschi M, Johnson ZL, Lee SY. Nature. 2017 May 4;545(7652):66-70. doi: 10.1038/nature22057. Epub 2017 Apr 17. 10.1038/nature22057 PubMed 28424521
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