-
PurposeExpresses CPT1A
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 100146 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonePHAGE-P-CMVt-N-HA-GAW
- Backbone size w/o insert (bp) 7800
- Total vector size (bp) 10038
-
Vector typeMammalian Expression, Lentiviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameCPT1A
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2268
-
Entrez GeneCPT1A (a.k.a. CPT1, CPT1-L, L-CPT1)
- Promoter CMV
-
Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
p7056 pHAGE-P-CMVt-N-HA-GAW-CPT1A was a gift from Peter Howley (Addgene plasmid # 100146 ; http://n2t.net/addgene:100146 ; RRID:Addgene_100146) -
For your References section:
Proteomic analysis and identification of cellular interactors of the giant ubiquitin ligase HERC2. Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, Harper JW, Howley PM. J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. Epub 2014 Dec 15. 10.1021/pr501005v PubMed 25476789