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p6329 pHAGE-P-CMVt-N-HA-GAW-HERC2-F5
(Plasmid #100139)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PHAGE-P-CMVt-N-HA-GAW
  • Backbone size w/o insert (bp) 7800
  • Total vector size (bp) 10650
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HERC2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2850
  • Mutation
    amino acids 3550-4500
  • Entrez Gene
    HERC2 (a.k.a. D15F37S1, MRT38, SHEP1, jdf2, p528)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p6329 pHAGE-P-CMVt-N-HA-GAW-HERC2-F5 was a gift from Peter Howley (Addgene plasmid # 100139 ; http://n2t.net/addgene:100139 ; RRID:Addgene_100139)

  • For your References section:

    Proteomic analysis and identification of cellular interactors of the giant ubiquitin ligase HERC2. Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, Harper JW, Howley PM. J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. Epub 2014 Dec 15. 10.1021/pr501005v PubMed 25476789
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