pSN32
(Plasmid
#100117)
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Purposeexpresses a chimeric KLF4-activator fusion
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100117 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLM-mCherry-KLF4
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Backbone manufacturerMichel Sadelain (Addgene plasmid # 23243)
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry-P2A-KLF4 fused to a 'weak' VP16 domain
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SpeciesH. sapiens (human)
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Entrez GeneKLF4 (a.k.a. EZF, GKLF)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSN32 was a gift from Mark Ptashne (Addgene plasmid # 100117 ; http://n2t.net/addgene:100117 ; RRID:Addgene_100117) -
For your References section:
OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071. 10.1016/j.celrep.2017.07.071 PubMed 28813671