Caspase-6
(Plasmid
#100102)
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PurposeBacterial expression of Caspase-6
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 100102 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET23b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCaspase-6
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SpeciesH. sapiens (human)
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Entrez GeneCASP6 (a.k.a. CSP-6, MCH2, caspase-6)
- Promoter T7
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Tag
/ Fusion Protein
- His (C terminal on backbone)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Caspase-6 was a gift from Jim Wells (Addgene plasmid # 100102 ; http://n2t.net/addgene:100102 ; RRID:Addgene_100102) -
For your References section:
Cacidases: caspases can cleave after aspartate, glutamate and phosphoserine residues. Seaman JE, Julien O, Lee PS, Rettenmaier TJ, Thomsen ND, Wells JA. Cell Death Differ. 2016 Oct;23(10):1717-26. doi: 10.1038/cdd.2016.62. Epub 2016 Jul 1. 10.1038/cdd.2016.62 PubMed 27367566