pETDuet_huCAD_ICADL (TevS)
(Plasmid #100098)

• Purpose: dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences
• Depositing Lab: Jim Wells
• Publication: Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8344-9. doi: 10.1073/pnas.1504141112. Epub 2015 Jun 23.

Backbone

• Vector backbone: pETDuet
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DNA fragmentation factor subunit alpha
• Species: H. sapiens (human)
• Mutation: two caspase cleavage sites (DETD-117 and DAVD-224) in ICAD were sequentially mutated to TEVP cleavable sequences
• Entrez Gene: CAD (a.k.a. CDG1Z, DEE50, EIEE50, GATD4)
• Entrez Gene: DFFA (a.k.a. DFF-45, DFF1, ICAD)
• Promoter: T7

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note- insertion of amino acids ENLYFG after AA117, and insertion of ENLYFQSG after AA224 in ICAD [Genbank sequence NP_004392.1.] are expected insertions. They make the ICAD variant recognizable and cleavable by TEVP.