ECFP-TevS-YPET
(Plasmid
#100097)
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PurposeFRET-based ECFP-YPET reporter that is cleaved by TEV
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 100097 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameECFP-TevS-YPET
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SpeciesSynthetic
- Promoter CMV
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Unknown
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ECFP-TevS-YPET was a gift from Jim Wells (Addgene plasmid # 100097 ; http://n2t.net/addgene:100097 ; RRID:Addgene_100097) -
For your References section:
Activation of specific apoptotic caspases with an engineered small-molecule-activated protease. Gray DC, Mahrus S, Wells JA. Cell. 2010 Aug 20;142(4):637-46. doi: 10.1016/j.cell.2010.07.014. 10.1016/j.cell.2010.07.014 PubMed 20723762
