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PurposeSNIPer plasmid, C terminal TEV fused to FKBP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100096 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepQCXIH
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Vector typeMammalian Expression, Retroviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameC-TEV
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Alt nameNIa tobacco etch virus protease
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MutationC terminal domain
- Promoter CMV
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Tags
/ Fusion Proteins
- Myc (N terminal on insert)
- FKBP (N terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FKBP-C-TEV 219 was a gift from Jim Wells (Addgene plasmid # 100096 ; http://n2t.net/addgene:100096 ; RRID:Addgene_100096) -
For your References section:
Engineered cellular gene-replacement platform for selective and inducible proteolytic profiling. Morgan CW, Diaz JE, Zeitlin SG, Gray DC, Wells JA. Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8344-9. doi: 10.1073/pnas.1504141112. Epub 2015 Jun 23. 10.1073/pnas.1504141112 PubMed 26106156