pET28-his-3C_Tau352
(Plasmid #100092)

• Purpose: Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag
• Depositing Lab: Nikolai Sluchanko
• Publication: Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi: 10.1371/journal.pone.0178933. eCollection 2017.

Backbone

• Vector backbone: pET28-his-3C
• Backbone size w/o insert (bp): 5231
• Total vector size (bp): 6343
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fetal microtubule-associated protein tau
• Alt name: Tau
• Alt name: MAP-Tau
• Species: H. sapiens (human)
• Mutation: wild type sequence
• Entrez Gene: MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
• Promoter: T7
• Tag / Fusion Protein:
  • His6 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • pET28a+His_3C_TauWT.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 3C site is introduced in front of Tau sequence for easy purification by subtractive IMAC