G9A[SET]-dCas9
(Plasmid #100089)

• Purpose: Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
• Depositing Lab: David Segal
• Publication: O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):9901-9916. doi: 10.1093/nar/gkx578.

Backbone

• Vector backbone: pcDNA3.3-TOPO
• Backbone manufacturer: ThermoFisher
• Backbone size w/o insert (bp): 5407
• Total vector size (bp): 11068
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G9A
• Alt name: EHMT2
• Species: H. sapiens (human)
• Mutation: G9A[SET] includes aa 829-1209
• GenBank ID: NP_006700.3
• Entrez Gene: EHMT2 (a.k.a. BAT8, C6orf30, G9A, GAT8, KMT1C, NG36)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3XFLag-NLS-G9A[SET]-dCas9-NLS

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: polyA TK-R

Resource Information

• Supplemental Documents:
  • G9A[SET]-iCas9v2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry