dCas9-FOG1[N+C]
(Plasmid #100085)

• Purpose: dCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
• Depositing Lab: David Segal
• Publication: O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):9901-9916. doi: 10.1093/nar/gkx578.

Backbone

• Vector backbone: pcDNA3.3-TOPO
• Backbone manufacturer: ThermoFisher
• Backbone size w/o insert (bp): 5407
• Total vector size (bp): 10198
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FOG1(aa 1-45)
• Alt name: ZFPM1
• Species: H. sapiens (human)
• Insert Size (bp): 135
• GenBank ID: AAN45858.1
• Entrez Gene: ZFPM1 (a.k.a. FOG, FOG1, ZC2HC11A, ZNF408, ZNF89A)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3XFLag-NLS-FOG1-dCas9-FOG1-NLS

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: polyA TK-R

Resource Information

• Supplemental Documents:
  • FOG[N+C] iCas9v2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry