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Addgene

pMT3-RhoGC E254D
(Plasmid #100033)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100033 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RhoGC E254D
  • Species
    B. emersonii
  • Mutation
    E254D
  • Promoter Adenovirus major late promoter
  • Tag / Fusion Protein
    • 1D4 (TETSQVAPA) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer TGC CTT TGA GGG TGG CCG
  • 3′ sequencing primer GCC GTC AAG TTT GGC GCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alanine insertion at the second position of the RhoGC amino acid sequence does not affect function. Mutation numbering is relative to the wild type reference sequence [KF309499].

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT3-RhoGC E254D was a gift from Daniel Oprian (Addgene plasmid # 100033 ; http://n2t.net/addgene:100033 ; RRID:Addgene_100033)

  • For your References section:

    Expression, purification, and spectral tuning of RhoGC, a retinylidene/guanylyl cyclase fusion protein and optogenetics tool from the aquatic fungus Blastocladiella emersonii. Trieu MM, Devine EL, Lamarche LB, Ammerman AE, Greco JA, Birge RR, Theobald DL, Oprian DD. J Biol Chem. 2017 Jun 23;292(25):10379-10389. doi: 10.1074/jbc.M117.789636. Epub 2017 May 4. 10.1074/jbc.M117.789636 PubMed 28473465
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