pMT3-RhoGC E254D
(Plasmid
#100033)
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PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100033 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMT3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRhoGC E254D
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SpeciesB. emersonii
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MutationE254D
- Promoter Adenovirus major late promoter
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Tag
/ Fusion Protein
- 1D4 (TETSQVAPA) (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer TGC CTT TGA GGG TGG CCG
- 3′ sequencing primer GCC GTC AAG TTT GGC GCG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Alanine insertion at the second position of the RhoGC amino acid sequence does not affect function. Mutation numbering is relative to the wild type reference sequence [KF309499].
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMT3-RhoGC E254D was a gift from Daniel Oprian (Addgene plasmid # 100033 ; http://n2t.net/addgene:100033 ; RRID:Addgene_100033) -
For your References section:
Expression, purification, and spectral tuning of RhoGC, a retinylidene/guanylyl cyclase fusion protein and optogenetics tool from the aquatic fungus Blastocladiella emersonii. Trieu MM, Devine EL, Lamarche LB, Ammerman AE, Greco JA, Birge RR, Theobald DL, Oprian DD. J Biol Chem. 2017 Jun 23;292(25):10379-10389. doi: 10.1074/jbc.M117.789636. Epub 2017 May 4. 10.1074/jbc.M117.789636 PubMed 28473465