pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40
(Plasmid
#100030)
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PurposeAAV expression of ArcLightD
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100030 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameArcLightD
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SpeciesSynthetic
- Promoter CBA
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Tag
/ Fusion Protein
- 2A-mCherry (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Penn Vector Core p2699
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40 was a gift from Lawrence Cohen (Addgene plasmid # 100030 ; http://n2t.net/addgene:100030 ; RRID:Addgene_100030) -
For your References section:
Monitoring brain activity with protein voltage and calcium sensors. Storace DA, Braubach OR, Jin L, Cohen LB, Sung U. Sci Rep. 2015 May 13;5:10212. doi: 10.1038/srep10212. 10.1038/srep10212 PubMed 25970202