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Addgene

pFX-Pickles 2.34NES
(Plasmid #100028)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100028 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFX
  • Total vector size (bp) 6128
  • Vector type
    Mammalian Expression ; pFX vector is derived from pCMV vector.

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha and JM109 are suitable growth strains.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pickles-2.34NES
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2101
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFX-Pickles 2.34NES was a gift from Yusuke Ohba (Addgene plasmid # 100028 ; http://n2t.net/addgene:100028 ; RRID:Addgene_100028)

  • For your References section:

    Improved FRET Biosensor for the Measurement of BCR-ABL Activity in Chronic Myeloid Leukemia Cells. Horiguchi M, Fujioka M, Kondo T, Fujioka Y, Li X, Horiuchi K, O Satoh A, Nepal P, Nishide S, Nanbo A, Teshima T, Ohba Y. Cell Struct Funct. 2017 Feb 2;42(1):15-26. doi: 10.1247/csf.16019. Epub 2016 Dec 8. 10.1247/csf.16019 PubMed 27928132
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