pcDNA3.2-DEST-MatryoshCaMP6s
(Plasmid #100025)

• Purpose: Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.
• Depositing Lab: Wolf Frommer
• Publication: Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10.1038/s41467-017-00400-2.

Backbone

• Vector backbone: pcDNA3.2/V5-DEST
• Backbone manufacturer: Thermo Fisher Scientific
• Backbone size w/o insert (bp): 5490
• Total vector size (bp): 7449
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MatryoshCaMP6s
• Alt name: GCaMP6s-LSSmOrange
• Alt name: MCaMP6s
• Insert Size (bp): 1959
• Promoter: CMV
• Tag / Fusion Protein:
  • V5 Epitope Tag (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • pcDNA3-2-DEST-MatyroshCaMP6s.gb
• A portion of this plasmid was derived from a plasmid made by: The GCaMP6s portion was derived from Addgene plasmid #40753 (Douglas Kim).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry